Application Notes describes the culture of specific lines, production of virus and specific technical solutions.
100 |
Oxygen Limitations in Small Spinner Vessels. Increasing spinner size from 50 to 250 ml reduces cell concentrations 50%. |
| 101 |
Large Scale Harvesting of Cells. More than 90% of the cells are recovered by a modified procedure. |
| 102 |
Growth of r-CHO in 1, 4 and 10 litre Fermentors. Production was scaled-up and maintained for more than 2 months. |
| 103 |
Critical Parameters for the Cultivation of r-CHO Cells. Expression of recombinant protein is strongly influenced by dissolved oxygen concentration. |
| 104 |
Growth of r-CHO Cells in Serum-free Medium. CultiSpher’s porous structure makes long-term serum-free culture possible. |
| 105 |
Vesicular Stomatitis Virus(VSV) Production by Vero Cells. Compared to solid microcarriers almost 10 times higher yields of virus were obtained. |
| 106 |
Growth of BHK 21 Cells in Medium with Reduced Serum. Growth of BHK 21 cells was maintained at reduced serum levels. |
| 107 |
Influence of Dissolved Oxygen Concentration on Cell Growth. Dissolved oxygen is the most critical parameter in spinner cultures. |
| 108 |
Optimisation of Microcarrier Concentration. Almost 10 x 109 CHO cells can be grown on 1 g of CultiSpher-G. |
| 109 |
Effect of Inoculum Size on Growth Span. Microcarrier cultures with CultiSpher can be scaled-up in steps of 400 times. |
| 110 |
Effect of Microcarrier Concentration on Cell Yield. Optimum microcarrier concentration must be determined for each system. |
| 111 |
Serial Cultivation of CHO-K1 Cells. Cells harvested from CultiSpher is ideal for scale-up purposes. |
| 112 |
Growth of MDCK Cells. MDCK cells can be cultured to high densities and harvested with quantitative yields. |
| 113 |
Growth of Recombinant and Non-recombinant CHO-cells. After a prolonged growth phase almost identical cell yields were obtained. |
| 114 |
Growth of BHK-21 Cells. BHK cells attached and grew efficiently on CultiSpher-G. |
| 115 |
Growth of Vero and GMK Cells. High yields of both these cell lines were obtained. A modified procedure for cell counting must be used. |
| 116 |
Growth of HeLa Cells. Large
numbers of HeLa cells were produced and collected
continuously.
|
