References

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A collection of scientific literature describing the use of CultiSpher microcarriers. Please contact us if you have additions to this list.

2016
  Inter-microcarrier transfer and phenotypic stability of stem cell-derived Schwann cells in stirred suspension bioreactor culture

Volume 113, Issue 2 Biotechnology and Bioengineering (2016),  113, 393-402

DOI: 10.1002/bit.25813

 

Recent advances in serum-free microcarrier expansion of mesenchymal stromal cells: Parameters to be optimized

Biochemical and Biophysical Research Communications (2016), 473, 769-773

doi:10.1016/j.bbrc.2015.09.078

 

Systematic microcarrier screening and agitated culture conditions improves human mesenchymal stem cell yield in bioreactors

Biotechnology Journal (2016), 11(4), 473-486

DOI: 10.1002/biot.201400862

 

Centimeter-sized biomimetic bone constructs fabricated via CBD-BMP2-collagen microcarriers and BMSC-gelatin microspheres

J. Mater. Chem. B, 2016,4, 461-470

DOI: 10.1039/C5TB02048D

 

Applications of human skin in vitro

Lönnqvist, Susanna, Doctoral thesis (2016)

DOI: 10.3384/diss.diva-123313

 

In vitro expansion of human cardiac progenitor cells: exploring 'omics tools for characterization of cell-based allogeneic products

Translational Research (2016), 171, 96–110.e3

doi:10.1016/j.trsl.2016.02.001

 

Fibroblast Differentiation and Models of Human Skin

Rakar, Jonathan, Doctoral thesis (2016)

DOI: 10.3384/diss.diva-125925

 

Biomaterials control of pluripotent stem cell fate for regenerative therapy

Progress in Materials Science (2016), 82, 234-293

doi:10.1016/j.pmatsci.2016.05.003

 

Nanostructured injectable cell microcarriers for tissue regeneration

Nanomedicine (2016) 11(12), 1611-1628

DOI 10.2217/nnm-2016-0083

 

Endogenous human skin equivalent promotes in vitro morphogenesis of follicle-like structures

Biomaterials (2016), 101, 86-95

doi:10.1016/j.biomaterials.2016.05.047

 

Toward a scalable and consistent manufacturing process for the production of human mesenchymal stem cells

CELL & GENE THERAPY INSIGHTS CELL & GENE THERAPY (2016), 127-140

DOI:10.18609/cgti.2016.008

 

Large-Scale Expansion and Differentiation of Mesenchymal Stem Cells in Microcarrier-Based Stirred Bioreactors

Part of the series Methods in Molecular Biology (2016), 1-16

DOI:10.1007/7651_2015_314

 

Inter-microcarrier transfer and phenotypic stability of stem cell-derived Schwann cells in stirred suspension bioreactor culture

Biotechnology and Bioengineering (2016), 113(2), 393–402

DOI: 10.1002/bit.25813

 

Primary skeletal muscle cells cultured on gelatin bead microcarriers develop structural and biochemical features characteristic of adult skeletal muscle

Cell Biology International (2016), 40, 364–374

DOI: 10.1002/cbin.10565
 

The Potential Application of Biomaterials in Cardiac Stem Cell Therapy

Current Medicinal Chemistry (2016), 23(6), 589-602

 

Centimeter-sized biomimetic bone constructs fabricated via CBD-BMP2-collagen microcarriers and BMSC-gelatin microspheres

J. Mater. Chem. B, 2016, 4, 461-470

DOI: 10.1039/C5TB02048D

 

Stirred tank bioreactor culture combined with serum-/xenogeneic-free culture medium enables an efficient expansion of umbilical cord-derived mesenchymal stem/stromal cells

Biotechnol. J. (2016)

DOI: 10.1002/biot.201500532

 

Cell interactions under control of surface substrate

J Appl Tis. Eng. (2016), 3(1), 10-28

2015
 

Feasibility of human hair follicle-derived mesenchymal stem cells/CultiSpher®-G constructs in regenerative medicine

Cell Tissue Res (2015) 362: 69. doi:10.1007/s00441-015-2182-z

 

3D tissue-like assemblies: A novel approach to investigate virus–cell interactions

Methods Related to Molecular Virology (2015), 90, 76–84

doi:10.1016/j.ymeth.2015.05.010

 

Bioreactor concepts for cell culture-based viral vaccine production

Expert Review of Vaccines (2015), 14(9), 1181-1195

DOI:10.1586/14760584.2015.1067144

 

Gelatine Microspheres Support Direct Intramyocardial Delivery of Induced Pluripotent Stem Cell-Derived Cardiomyocytes

J Stem Cell Res Ther 2015, 5:9

http://dx.doi.org/10.4172/2157-7633.1000306

 

Large-Scale Expansion and Differentiation of Mesenchymal Stem Cells in Microcarrier-Based Stirred Bioreactors

Methods in Molecular Biology (2015)
DOI 10.1007/7651_2015_314

 

Stirred tank bioreactor culture combined with serum-/xenogeneic-free culture medium enables an efficient expansion of umbilical cord-derived mesenchymal stem/stromal cells

Biotechnology J. (2015)

DOI: 10.1002/biot.201500532

 

Past, present, and future of microcarrier-based tissue engineering

Journal of Orthopaedic Translation (2015), 3(2), 51-57

 

Suspension-Expansion of Bone Marrow Results in Small Mesenchymal Stem Cells Exhibiting Increased Transpulmonary Passage Following Intravenous Administration

Tissue Engineering Part C: Methods.(2015), 21(7), 683-692

 

Biodegradable Gelatin Microcarriers Facilitate Re-Epithelialization of Human Cutaneous Wounds - An In Vitro Study in Human Skin (2015)

http://dx.doi.org/10.1371/journal.pone.0128093

 

Large-scale expansion of Wharton’s jelly-derived mesenchymal stem cells on gelatin microbeads, with retention of self-renewal and multipotency characteristics and the capacity for enhancing skin wound healing

Stem Cell Research & Therapy (2015),  6:38

DOI: 10.1186/s13287-015-0031-3

 

Cell-laden Polymeric Microspheres for Biomedical Applications

Trends in Biotechnology (2015),33 , 653–666

doi:10.1016/j.tibtech.2015.09.003

 

Collagen-low molecular weight hyaluronic acid semi-interpenetrating network loaded with gelatin microspheres for cell and growth factor delivery for nucleus pulposus regeneration

Acta Biomaterialia (2015), 20, 10–21

doi:10.1016/j.actbio.2015.03.041

 

Application of biomaterials to advance induced pluripotent stem cell research and therapy

The EMBO Journal (2015)

DOI 10.15252/embj.201490756 2015
2014
 

Hepatic differentiation of human embryonic stem cells on microcarriers

Journal of Biotechnology (2014), 174, 39-48

 

Stem cell bioprocess engineering towards cGMP production and clinical applications

Cytotechnology (2014), 66(5), 709-722

 

Innovative tissue models for drug discovery and development

Advanced Drug Delivery Reviews (2014), 69-70, 1-18

 

Critical analysis of 3-D organoid in vitro cell culture models for high-throughput drug candidate toxicity assessments

doi:10.1016/j.addr.2014.02.008

 

Perfusion culture-induced template-assisted assembling of cell-laden microcarriers is a promising route for fabricating macrotissues

Biotechnology Journal (2014), 9(11), 1425-143

DOI: 10.1002/biot.201400238

 

Induced Pluripotent and Mesenchymal Stem Cells as a Promising Tool for Articular Cartilage Regeneration

J Cell Sci Ther 2014, 5:4

http://dx.doi.org/10.4172/2157-7013.1000172

 

An investigation in the in vitro and in vivo use of microcarrier beads to support keratinocytes and the effect on wound contraction

 

http://discovery.ucl.ac.uk/id/eprint/1450740

 

Macroporous microcarriers for introducing cells into a microfluidic chip

Lab Chip (2014), 14, 3502-3504

DOI: 10.1039/C4LC00693C

 

Cellularized Microcarriers as Adhesive Building Blocks for Fabrication of Tubular Tissue Constructs

Annals of Biomedical Engineering (2014), 42(7), 1470-1481

doi:10.1007/s10439-013-0883-6

 

Maximizing mouse embryonic stem cell production in a stirred tank reactor by controlling dissolved oxygen concentration and continuous perfusion operation

Biochemical Engineering Journal (2014), 85, 81-90

doi:10.1016/j.bej.2013.11.014

 

Investigation of Growth Conditions for the Expansion of Porcine Mesenchymal Stem Cells on Microcarriers in Stirred Cultures

Applied Biochemistry and Biotechnology (2014), 172(2), 1004–1017

DOI: 10.1007/s12010-013-0586-3

 

Ectopic Osteogenesis of Macroscopic Tissue Constructs Assembled from Human Mesenchymal Stem Cell-Laden Microcarriers through In Vitro Perfusion Culture

PLOS 2014 http://dx.doi.org/10.1371/journal.pone.0109214

 

Improved expansion of human bone marrow-derived mesenchymal stem cells in microcarrier-based suspension culture

Journal of Tissue Engineering and Regenerative Medicine (2014), 8(3), 210-225

DOI: 10.1002/term.1515,

 

Fabrication of viable centimeter-sized 3D tissue constructs with microchannel conduits for improved tissue properties through assembly of cell-laden microbeads

Journal of Tissue Engineering and Regenerative Medicine (2014), 8(6), 493-504

DOI: 10.1002/term.1554

2013
 

Allogeneic cell therapy bioprocess economics and optimization: Single-use cell expansion technologies

Biotechnol. Bioeng. DOI 10.1002/bit.25008

 

Cytotoxicity of Ferulic Acid on T24 Cell Line Differentiated by Different Microenvironments

Biomed Res. Int. 2013, 1-7, 2013 DOI 10.1016/j.abb.2006.03.003

 

Cellularized Microcarriers as Adhesive Building Blocks for Fabrication of Tubular Tissue Constructs

Ann Biomed Eng. DOI 10.1007/s10439-013-0883-6

 

Basic science and clinical application of platelet-rich plasma for cartilage defects and osteoarthritis: a review

Osteoarthritis and Cartilage, 21, 1627-1637, 20123

 

Enhancing the Mesenchymal Stem Cell Therapeutic Response: Cell Localization and Support for Cartilag

Tissue Engineering Part B DOI: 10.1089/ten.teb.2012.0101

 

Impact of Growth Factors on the Proliferation of Ear Mesenchymal Stem Cells on Porous Microcarriers

ESACT Proceedings 5 DOI10.1007/978-94-007-0884-6_27

 

Improved expansion of human bone marrow-derived mesenchymal stem cells in microcarrier-based suspension

Tissue engineering Regen Med http://onlinelibrary.wiley.com/doi/10.1002/term.1515

 

Investigation of Growth Conditions for the Expansion of Porcine Mesenchymal Stem Cells on Microcarriers in Stirred Cultures

Appl. Biochem. Biotechnol DOI 10.1007/s12010-013-0586-3

 

Large-Scale Expansion of Mouse Embryonic Stem Cells on Microcarriers

Embryonic Stem Cell Therapy for Osteo-Degenerative DiseasesMethods and Protocols DOI 10.1007/978-1-60761-962-8_8

 

Microcarrier Culture for Efficient Expansion and Osteogenic Differentiation of Human Fetal Mesenchymal Stem Cells

Bioresearch open access 2, 84-97, 2013

 

Three-Dimensional Normal Human Neural Progenitor Tissue-Like Assemblies: A Model of Persistent Varicella-Zoster Virus Infection

PLOS Pathogen doi:10.1371/journal.ppat.1003512

 

Scale-up of human mesenchymal stem cell culture: current technologies and future challenges

Current opinion in chem. Eng. 2, 8-16, 2013

 

Application of human mesenchymal and pluripotent stem cell microcarrier cultures in cellular therapy: Achievements and future direction

Biotechnol advances doi:10.1016/j.biotechadv.2013.03.006

 

Growth, metabolic activity, and productivity of immobilized and freely suspended CHO cells in perfusion culture

Biotechnol Applied Biochem DOI: 10.1002/bab.1103

 

Stem cell engineering in bioreactors for large-scale bioprocessing Engineering

Llife sci DOI: 10.1002/elsc.201300013

 

Modulation of mesenchymal stem cell actin organization on conventional microcarriers for proliferation and differentiation in stirred bioreactors

J Tissue Eng Regen Med 7, 537-551, 2013

 

Bone grafts engineered from human adipose-derived stem cells in dynamic 3D-environment

Biomaterials 34, 1004-1017, 2013

 

Multiple phosphoryable sites in the Zaire Ebolavirus nucleoprotein evidenced by high resolution tandem mass spectrometry

J. Virol Meth. 187, 159-165, 2013

 

Osteogenically-induced human dermal fibroblasts as atool to regenerate bone

J. Plastic Surg. and Hand. Surg. 47, 8-13, 2013

2012
 

Wound Contraction Is Significantly Reduced by the Use of Microcarriers to Deliver Keratinocytes and Fibroblasts in an In Vivo Pig Model of Wound Repair

Tissue Engineering Part A. 18, 587-597, 2012

 

Enhancing the Mesenchymal Stem Cell Therapeutic Response: Cell Localization and Support for Cartilage Repair

Tissue Engineering Part B: Reviews DOI 10.1089/ten.teb.2012.0101

 

Optimization of culture of mesenchymal stem cells: a comparison of conventional plate and microcarrier cultures

Cell Proliferation 45, 430-437, 2012

 

Development of bone marrow mesenchymal stem cell culture in vitro

China Med. J. 125, 1650-1655, 2012

 

Large-scale production of human mesenchymal stem cells for clinical applications

Biotechnol Appl. Biochem 59, 106-120, 2012

 

Modulating and modeling aggregation of cell-seeded microcarriers in stirred culture system for macrotissue

J Biotechnol 150, 438-446, 2012

 

Engineered dermal equivalent tissue in vitro by assembly of microtissue precursors

Acta Biomaterialia 6, 2548-2553, 2012

 

Process engineering of human pluripotent stem cells for clinical application

Trends Biotechnol 30, 350-359,2012

 

Fabrication of viable centimeter-sized 3D tissue construct with microchannel conduits for improved tissue properties through assembly of cell-laden microbeads

J. Tissue Eng, Regen. Med. DOI 10.1002/term.1554

 

Improved expansion of human bone marrow-derived mesenchymal stem cells in microcarrier-based suspension culture

J. Tissue Eng, Regen. Med. DOI 10.1002/term.1514

 

Optimization of culture of mesenchymal stem cells: a comparison of conventional plate and microcarrier culture

Cell Proliferation 45, 430-437, 2012

 

Optimization and scale-up of human endometrial multipotent mesenchymal stromal cells: potential for clinical application

Tissue Engineering part C DOI 10.1089/ten.tec.2011.0718

 

Adaptive metabolic resonse to 4 weeks of sugar-sweetened beverage consumption in healthy, lightly active individuals and chronic high glucose availability in primary human myotubes

Eur. J. Nutr. DOI 10.1007/s00394-012-0401-x

 

Process engineering of human pluriopotent stem cells for clinical application

Trends in Biotechnol. 30, 350-359, 2012

 

Attachment of embryonic stem cells-derived cardiomyocytes in CultiSpher-S microcarriers by using spinner flask

QScience Proc, DOI 10.5339/qproc.2012.stem.1.49

 

Development of bone marrow mesenchymal stem cell culture in vitro

Cina Med. J. 125, 1650-1655, 2012

 

SK-HEP cells and lentiviral vector for production of human recombinant factor VIII

Biotechnol. Lett. 34, 1435-1443, 2012

 

Closed system isolation and scalable expansion of human placental mesenchymal cells

Biotechnol. Bioeng, DOI 10.1002/bit.24425

2011
 

Adult human articular chondrocytes in a microcarrier-based culture system: expansion and redifferentiation

J. Orthopaedic Res. 29, 539-546, 2011

 

Towards human central nervous system in vitro models for preclinical researc: strategies for 3D neural cell culture

DOI 10.1186/1753-6561-5-S8-P53

 

Contolled expansion and differentiation of mesenchymal dtem cells in a microcarrier based stirred bioreactor

DOI 10.1186/1753-6561-5-S8-P55

 

Cell expansion of human articular chondrocytes on macroporous gelatine scaffolds - impact of microcarrier selection on cell proliferation

Biomed. Mater. 6 DOI 10.1088/1748-6041/6/6/065001

 

Towards a clinical-grade expansion of mesenchymal stem cells from human sources: a microcarrier-based culture system under xeno-free conditions

Tissue Engineering part C 17, 2011 DOI 10.1089/ten.tec.2011.0255

 

Use of macroporous gelatine spheres as a biodegradable scaffold for guided tissue regeneration of healthy dermis in humans: an in vivo study

Brit. J. Plast. Surg. 63, 848-857, 2011

 

Facile synthesis [5-13C-4-2H2]-L-Glutamine for hyperpolarized MRS imaging of cancer cell metabolism

Acad. Radiol. 18, 932-939, 2011

 

Cranofacial defect regeneration using engineered bone marrow mesenchymal stromal cells

J. Biomed. Mater. Res. A DOI:10.1002/jbm.a.33155

 

Scale-up of mouse embryonic stem cell expansion in stirred bioreactors

Biotechnol Prog.  DOI 10.1002/btpr.658

 

A modular approach to the engineering of centimeter-sized bone tissue construct with human amniotic mesenchymal stem cells-laden microcarriers

Biomaterials DOI 10.1016/j.biomaterials.2011.06.054

 

Critical microcarrier properties affecting the expansion of undifferantiated human embryonic stem cells

Stem Cell Res. 7, 97-11, 2011

 

Effects of oxygen and culture system on in vitro propagation and redifferantiation osteoarthritic human articular chondrocytes

Cell Tissue Res. 128, 1-15,2011

 

Large-scale production of murine embryonic stem cell-derived osteoblasts and chondrocytes onh microcarriers in serum-free media

Biomaterials DOI 10.1016/j.biomaterials.2011.04.015

 

Intrastriatal transplantation of microcarrier-bound human restinal pigment epithelial cells versus sham surgery in patients with advanced Parkinson´s disease: a double-blind randomized, controlled trial

The Lancet Neurology 10, 509-519, 2011

 

Efficient suspension bioreactor expansion of murine embryonic stem cells in serum-free medium

Biotechnol. Progr. 27, 811-823, 2011

 

Gene regulating mediating fiber-type transformation in skeletal muscle cells is partly glucose- and ChREBP-dependent

BBA - Molecular Cell Res. 1813, 377-389, 2011

 

Ex vivo expansion of human mesenchymal stem cells on microcarriers

Meth. Mol. Biol. 698, 189-198, 2011

 

Transplantation of acellular dermis and keratinocytes cultured on porous biodegradable microcarriers into full-thickness skin injuries on athymic rats

Burns 37, 99-108, 2011

 

Microcarriers and their potential in tissue regeneration

Tiisue Engineering part B 17, 71-80, 2011

 

Stem cell-based dendritic cell vaccine development: a review with emphasis on lung cancer treatment

J. Hematological Malignances DOI 10.5430/jhm.vn1p351

 

An emerging paradigm in tissue engineering: from chemical engineering to developmental engineering for bioartificial tissue formation through a series of unit operations that simulate the in vivo successive developmental stages

Ind. Eng. Chem. Res. 50, 482-522, 2011

 

Functional cells cultured on microcarriers for use in regenerative medicine research

Cell Transplantation 20, 49-62, 2011

 

Cell survival and poliferation after encapsulation in a chemically modified pluronic F127 hydrogel

J. Biomaterials Applications DOI 10.1177/0885328211427774

 

Wound contraction is significantly reduced by the use of microcarriers to deliver keratinocytes and fibroblasts in an in vivo pig model of skin repair and regeneration

Tissue Engineering DOI 10.1089/ten.tea.2011.0258

2010
 

Isolation and expansion of osteogenic cells from bone marrow and in vivo testing at ectopic sites

J. Stomat. Occ. Med. 3, 200-203, 2010

 

Modulating and modeling aggregation of cell-seeded microcarriers in stirred culture systems for macrotissue engineering

J. Biotechnology 150, 438-446, 2010

 

The effect of simulated microgravity on human mesenchymal stem cells cultured in an osteogenic differentiation system: a bioinformatics study

Tissue Engineering part A 16, 3403-3412, 2010

 

Periodontal regeneration using engineered bone marrow mesenchymal stromal cells

Biomaterials 31, 8574-8582, 2010

 

Large-scale expansion of mouse embryonic stem cells on microcarriers

Methods in Molecular Biology 690, 121-134, 2010

 

Cell proliferation of human bone marrow mesenchymal stem cells on biodegradable microcarriers enhances in vitro differentiation potential

Cell Proliferation 43, 445-456, 2010

 

Three-dimensional culture systems for the expansion of pluripotent embryonic stem cells

Biotech. Bioeng. 107, 683-695, 2010

 

Development of a vaccine candidate against heartwater

Cells and culture, Esact proceedings 4, 703-710, 2010

 

Influence of culture parameters on ear mesenchymal stem cells expanded on microcarriers

J. Biotechnol. 150, 149-160, 2010

 

Engineering three-dimensional cartilage- and bone-like tissues using human dermal fibroblasts and macroporous gelatine microcarriers

J. Plastic, Reconstructive & Aesthetic Surgery 63, 1036-1046, 2010

 

Passive mechanical forces upregulate the fast myosin heavy chain IId/x via integrin and p38 MAP kinase activation in a primary muscle cell culture

Am. J. Physiol. Cell Physiol 298, C910-C920, 2010

 

Engineering dermal equivalent tissue in vitro by assembly of microtissue precursors

Acta Biomaterialia 6, 2548-2553, 2010

 

Biodegradable and injectable cure-on-demand polyurethan scaffolds for regeneration of articular cartilage

Acta Biomaterialia 6, 3471-3481, 2010

 

Slow cooling cryopreservation of cell-microcarrier constructs

Cells Tissues Organs, 192, 177-186, 2010

 

Use of macroporous gelatine spheres as a biodegradable scaffold for guided tissue regeneration of healthy dermis in humans: An in vivo study

Journal of Plastic, Reconstructive & Aesthetic Surgery , Volume 63, 848-857, 2010

 

Evaluation of Bone Regeneration with an Injectable, In Situ Polymerizable Pluronic ® F127 Hydrogel Derivative Combined with Autologous Mesenchymal Stem Cells in a Goat Tibia Defect Model

Tissue Engineering Part A. February 2010, 16(2): 617-627.

 

Maximizing the ex vivo expansion of human mesenchymal stem cells using a microcarrier-based stirred culture system.

J. Biotechnol., 146(4), 194-7, 2010

2009200
 

Human Dermal Fibroblasts: A Potential Cell Source for Endothelialization of Vascular Grafts

Annals of Vascular Surgery , Volume 23,663-674, 2009

 

Human articular chondrocytes on macroporous gelatin microcarriers form structurally stable constructs with blood-derived biological glues in vitro

J. Tissue. Eng. Regen. Med. 3, 450-60, 2009

 

Functional and phenotypic characterization of human keratinocytes expanded in microcarrier culture

J. Biomed.Mater.Res. 88A, 184-94, 2009

 

Global Transcriptome Analysis in Mouse Calvarial Osteoblasts Highlights Sets of Genes Regulated by Modeled Microgravity and Identifies A "Mechanoresponsive Osteoblast Gene Signature"

J. Cell. Biochem. 107, 240-52, 2009

 

The development of photochemically crosslinked native fibrinogen as a rapidly formed and mechanically strong surgical tissue sealant

Biomaterials 30, 2059-2065, 2009

 

Ear mesenchymal stem cells: An efficient adult multipotent cell population fit for rapid and scalable expansion

J. Biotechnol. 139, 291-299, 2009

20082008
 

Attachment and growth of human embryonic stem cells on microcarriers

J. Biotechnol. 138, 24-32, 2008

 

Entrapment of Embryonic Stem Cells-Derived Cardiomyocytes in Macroporous Biodegradable Microspheres: Preparation and Characterization

Cell. Physiol.Biochem. 22, 665-672, 2008

 

Metabolic transformation of rabbit skeletal muscle cells in primary culture in response to low glucose

BBA 1783, 813-825, 2008

 

Functional and phenotypic characterization of human keratinocytes expanded in microcarrier culture

J. Biomed. Mater. Res. 88A, 184-194, 2008

2007
 

Employing human keratinocytes cultured on macroporous gelatin spheres to treat full thickness-wounds: an in vivo study on athymic rats

Burns 33, 726-735, 2007

 

Mouse Embryonic Stem Cell Expansion in a Microcarrier-based Stirred Culture System

J. Biotechnology 132, 227-236, 2007

 

Collagenous Microbeds as a Scaffold for Tissue Engineering with Adipose-derived Stem Cells

Plast. Reconstr. Surg. 120, 441-4241, 2007

 

Comparison of Biodegradable Conduits within Aged Rat Sciatic Nerve Defects

Plast. Reconstr. Surg. 119, 1839-1851, 2007

 

Cost-effective Engineering of a Small-scale Bioreactor

Biotechnol. & Bioeng. 96, 401-407, 2007

 

Evaluating Kinetic and Physiological Features of rCHO-K1 Cells Cultured on Microcarriers for Production of a Recombinant Metalloprotease/Disintegrin

Electronic J. Biotechnology 10, 200-210, 2007

 

Macroporous Gelatine Spheres as Culture Substrate, Transplantation Vehicle, and Degradable Scaffold for Guided Regeneration of Soft Tissues. In Vivo Study in Nude Mice

J. Plast. Reconstr. Aesthet Surg. 60, 543-555, 2007

 

Ex Vivo Expansion of Rat Bone Marrow Mesenchymal Stromal Cells on Microcarrier Beads in Spin Culture

Biomaterials 28, 3110-3120, 2007

 

Gelatin-Based Microcarriers as Embryonic Stem Cell Delivery System in Bone Tissue Engineering: An In-Vitro Study

Biomacromolecules 8, 825-832, 2007

2006
 

Microcarriers in the Engineering of Cartilage and Bone

Trends in Biotechnol. 24, 299-304, 2006

 

Process Development for the Mass Production of Ehrlichia ruminantium

Vaccine 24, 1716-1725, 2006

 

High Yields of Autologous Living Dermal Equivalents Using Porcine Gelatin Microbeads as Microcarriers for Autologous Fibroblasts

Cell Transplantation 15, 445-451, 2006

 

A Novel Bioreactor Microcarrier Cell Culture System for High Yields of Proliferating Autologous Keratinocytes

Cell Transplantation 15, 435-443, 2006

 

Expansion of Chondroprogenitor Cells on Macroporous Microcarriers as an Alternative to Conventional Monolayer Systems

Biomaterials 27, 2970-9, 2006

2005
 

Encapsulation of osteoblast seeded microcarriers into injectable, photopolymerizable three-dimensional scaffolds based on D,L-lactide and epsilon-caprolactone

Biomacromolecules 6(3), 1608-1614, 2005

 

Evaluation of Polymer Scaffolds to be used in a Composite Injectable System for Intervertebral Disc Tissue Engineering

J. Biomed. Mater. Res. 74A, 32-39, 2005

 

Artificial Tumour Model Suitable for Monitoring 31P and 13C NMR Spectroscopic Changes During Chemotherapy-Induced Apoptosis in Human Glioma Cells

Magn. Res. Med. 54, 67-78, 2005

 

Evaluation of Polymer Scaffolds to be Used in a Composite Injectable System for Invertebral Disc Tissue Engineering

J. Biomed. Mater. Res. 74A, 32-39. 2005

 

Intrastriatal Implantation of Human Retinal Pigment Epithelial Cells Attached to Microcarriers in Advanced Parkinson Disease

Arch. Neurol. 62, 1833-1837, 2005

2004
 

Interactions Between Intracellular Chloride Concentrations, Intracellular pH and Energetic Status in Rat Lactotrope Cells in Primary Culture

Gen. Physiol. Biophys. 23, 195-208, 2004

 

Bioreactor Microcarrier Cell Culture System (Bio-MCCS) for Large-Scale Production of Autologous Melanocytes

Cell Transplantation 13, 809-816, 2004

 

PET Imaging of Implanted Human Retinal Pigment Epithelial Cells in the MPTP-induced Primate Model of Parkinson´s Disease

Exp. Neurol. 189, 362-368, 2004

 

Autologous Cultured Keratinocytes on Porcine Gelatin Microbeads Effectively Heal Cronic Venous Leg Ulcers

Wound Repair and Regen. 12:2, 148-156, 2004

 

Muti-channeled biodegradable polymer/CultiSpher composite nerve guides

Biomaterials 25, 1269-1278, 2004

 

Rabies Virus Production in High Vero Cell Density Cultures on Macroporous Microcarriers

Biotechnol. Bioeng. 85, 506-515, 2004

2003
 

Ca 2+ Transients Activate Calcineurin/NFATc1 and Initiate Fast-to-slow Transformation in a Primary Skeletal Muscle Culture

Am. J. Physiol. Cell Physiol. 285, C56-C63, 2003

 

Contribution of the Phosphorylable Complex I in the Growth Phase-Dependent Respiration of C6 Glioma Cells in Vitro

J. Bioenergetics Biomembranes 35, 439-450, 2003

 

Stereotaxic Intrastriatal Implantation of Human Retinal Pigment Epithelial (hRPE) Cells Attached to Gelatin Microcarriers: a Potential New Cell Therapy for Parkinson´s Disease

J. Neural. Transm. (Suppl.) 65, 215-227, 2003

 

Expansion of human nasal chondrocytes on macroporous microcarriers enhances redifferentiation

J. Biomaterials 24, 5153-5161, 2003

 

Using PC12 Cells to Evaluate Poly(caprolactone) and Collagenous Microcarriers for Application in Nerve Guide Fabrication

Biotechnol. Prog. 19, 1767-1775, 2003

 

ATP Generation in the Trypanosoma brucei Procyclic Form

J. Biol.Chem. 278:49, 49625-49635, 2003

2002

Ex vivo expansion of human umbilical cord blood hematopoietic progenitor cells in a novel three-dimensional culture system

Biotechnol. Lett. 24, 1421-1426, 2002

Na+/H+ exchange subtype 1 inhibition during exytacellular acidification and hypoxia in glioma cells

J. Neurochem. 80, 36-44, 2002

Microcarrier Culture of Lepidopteran Cell Lines: Implications for Growth and Recombinant Protein Production

Biotechnol. Progr. 18, 1345-1355, 2002,

Multinuclear NMR Studies of an Actively Dividing Artificial Tumour

Physiol. Res. 51, 49-58, 2002

Process Development for Fuctional Membrane Receptor Production in Mammalian Cells

Cytotechnology 38, 109-117, 2002

Fast-To-Slow Transformation and Nuclear Import/Export Kinetics of the Transcription Factor NFATc1 During Electrostimulation of Rabbit Muscle Cells in Culture

J. Physiol. 541.3, 835-847, 2002

2001

Effect of Ethanol on the Metabolism of Primary Astrocytes Studied by 13C- and 31P-NMR Spectroscopy

J. Neurosci. Res. 66:5, 803-811, 2001

Entrapment of Dispersed Pancreatic Islet Cells in CultiSpher-S Macroporous Gelatin Microcarriers: Preparation, In Vitro Characterization, and Microencapsulation

Biotech. Bioeng. 75:6, 741-744, 2001

Calcineurin Regulates Slow Myosin, but not Fast Myosin or Metabolic Enzymes, During Fast-To-Slow Transformation in Rabbit Skeletal Mucle Cell Culture

J. Physiol. 533.1, 215-226, 2001

2000

High-Resolution Elasticity Imaging for Tissue Engineering

IEE Transaction on Ultrasonics, Ferroelectrics and Frequency Control 47, 956-966, 2000

Development of a rapid and sensitive bioassay using human cells immobilized in macroporous microcarriers for on-site evaluation of environmental waters

Appl. Microbiol.Biotechnol. 54, 432-438, 2000

Cultivation of Immortalized Human Hepatocytes HepZ on Macroporous CultiSpher-G Microcarriers

Biotech. Bioeng. 68:1, 59-70, 2000

Culture and Function of Electrofusion-Derived Clonal Insulin-Secreting Cells Immobilized on Solid and Macroporous Microcarrier Beads

Bioscience Repoports 20:3, 167-176, 2000

Assessing a Disposable Bioreactor for Attachment-Dependent Cells

http://www.wavebiotech.com/pdfs/literature/biopharmfeb2000.pdf

1999

Production of reovirus type-1 and type-3 from Vero cells grown on solid and macroporous microcarriers

Biotechnol Bioeng. 62, 12-19 1999

An Integrated Strategy for the Process Development of a Recombinant Antibody-Cytokine Fusion Protein Expressed in BHK Cells

Appl. Microbiol. Biotechnol. 52, 345-353 1999

Cell Growth and Protein Formation on Various Microcarriers
Cytotechnology 29, 149-156 1999
Immobilization of Pancreatic Islet Cells with Preserved Secretory Potential
Appl. Microbiol. Biotechnol. 52, 652-653 1999
A Microcarrier-based Cell Culture Process for the Production of a Bovine Respiratory Syncytial Virus Vaccine
Cytotechnology 29, 135-148 1999
Intracranial Tolerability of Spheramine (Gelatin Microcarrier-bound Human Retinal Pigment Epithelial (hRPE) Cells) and Gelatin Microcarriers in Cynomolgus Monkey
Society for Neurosciences Abstracts Vol 25, Part 1 29th Annual Meeting, Miami beach, Fla, October 23-28, 1999

Growth of Human Tumour Cells in Macroporous Microcarriers Results in p53-Independent, Decreased Cisplatin Sensitivity Relative to Monolayers
Mol. Pharm. 55, 938-947 1999

Cultivation and Characterization of a New Immortalized Human Hepatocyte Cell Line, Hepz, for Use in an Artficial Liver Support System
Ann N Y Acad Sci. Jun 18;875,364-8 1999
1998
Performance Study of Perfusion Cultures for the Production of Single.Chain Urokinase.Type Plasminogen Activator (scu-PA) in a 2.5 l Spin-Filter Bioreactor
Bioprocess Eng. 19, 363-372 1998
Study of Different Cell Culture Conditions for the Production of a "Reshaped" MAB in NSO Cells
In New Developments and New Applications in Animal Cell Technology. Kluwer Academic Publishers. 51-53 1998
1997
Adult fast myosin pattern and Ca2+-induced slow myosin pattern in primary skeletal muscle culture
Proc. Natl. Acad. Sci. USA 94, 4205-4210 1997
Topgraphical Imaging of Macroporous Microcarriers
In Animal Cell Technology: Basic & Applied Aspects. Kluwer Academic Publishers. 8, 89-94 1997

Infiltration and Lysis of Tumour Cell Aggregates by Adherent Interleukin-2-Activated Natural Killer Cells is Distinct from Specific Cytolysis
Natural Immunity 15, 87-97 1996-97

Increasing GPI-Anchored Protein Harvest Concentrations from Suspension and Porous Microcarrier CHO Cell Cultures
Biotech. Bioeng. 55, 136-147 1997
Cell Propagation is Overestimated by the Crystal Violet Nuclei Counting Technique
In Animal Cell Technology. Kluwer Academic Publishers. 461-466 1997
Process Control of Antithrombin III Production by Near-infrared Spectroscopy
In Animal Cell Technolgy. Kluwer Academic Publishers. 417-421 1997
Immobilisation of Primary Brain Cells in Porous Microcarriers for On Line NMR Spectroscopy
In Animal Cell Technology. Kluwer Academic Publishers. 91-98 1997
Three-dimensional Cell Culture Systems Stabilize the Differentiation of Hepatocyte Cell Lines.
In Animal Cell Technology. Kluwer Academic Publishers. 115-119 1997
The Effect of Cell Culture Systems in the Phenotypic Stability in Primary Hepatocyte Cells
In Animal Cell Technology. Kluwer Academic Publishers. 145-149 1997
1996
Immobilization of Primary Astrocytes and Neurons for On-Line Monitoring of Biochemical Processes by NMR
Dev. Nerosci. 18, 478-483 1996
The Use of Macroporous Microcarriers for the Large-Scale Growth of V79 Cells Genetically Designed to Express Single Human Cytochrome P450 Isoenzymes and for the Characterization of the Expressed Cytochrome P450
Protein Expr. Purif. 8, 439-446 1996
Topographical Imaging of Macroporous Microcarriers Using Laser Scanning Confocal Microscopy
J. Fermen. Bioeng. 81, 437-444 1996
Confocal Laser Scanning Microscopy Examination of Cell Distribution in Macroporous Microcarriers
Biotechnol. Prog. 12, 398-402 1996
Growth and Radiation Responce of Cells Grown in Macroporous Gelatin Microcarriers(CultiSpher-G)
Brit. J. Cancer. Suppl. XXVII, S-78-S82 1996
Optimization of Physical Parameters for Cell Attachment and Growth on Macroporous Microcarriers
Biotech. Bioeng. 50, 627-635 1996
Two-dimensional Versus Three-dimensional Culture Systems: Effects on Growth and Productivity of BHK Cells
Biotech. Bioeng. 52, 429-432 1996
1995
The Use of Microcarrier Beads in Ion Transport NMR Studies of Perfused Cells
J.Magnetic Res. 108, 81.85 1995
Membrane Anchored Protein Production from Spheroid, Porous and Solid Microcarrier Chinese Hamster Ovary Cell Cultures
Biotech. Bioeng. 47, 550-556 1995
Influence of the Culture System Upon Growth and Productivity of Animal Cells in Stirred Systems
In Animal Cell technology: Developments towards the 21st Century. Kluwer Academic Publishers. 105-109 1995
Microcarrier Technology, Present Status and Perspective
In Animal Cell technology: Developments towards the 21st Century. Kluwer Academic Publishers. 899-904 1995
1994
Intraocular Bioassay of a Pronephric Tumour Cell Line Cultivated on Macroporous Microcarrier Beads
In Vitro Cell. Dev. Biol. 30A, 85-86 1994
Expansion of Hematopoietic Progenitor Cell Populations in Stirred Suspension Bioreactors of Normal Human Bone Marrow Cells
Bio/Technology. 14, 909-914 1994
Glycolipid Membrane Anchored Recombinant Protein Production from CHO Cells Cultures on Porous Microcarriers
Biotech. Bioeng. 44, 45-54 1994
Measurements of the Growth and Distribution of Mammalian Cells in a Hollow-Fiber Bioreactor Using Nuclear Magnetic Resonance Imaging
Bio/Technology 12, 75-78 1994
Cerebral Neurons, Skeletal Myoblasts, and Cardiac Muscle Cells Cultures on Macroporous Beads
Biotech. Bioeng. 43, 826-831 1994
Bead-to-bead Transfer of Chinese Hamster Ovary cells Using Macroporous Microcarriers
Cytotechnology 14, 67.80 1994
1993
High-level Expression of Recombinant Human Soluble Thrombmodulin in Serum-free Medium by Cho-K1 Cells
Appl. Microbiol. Biotechnol. 38, 520-525 1993
Evaluation of Anchorage-dependent Cell Propagation Systems for Production of Human Acetylcholinesterase by Recombinant 293 Cells
Cytotechnology 13, 115-123 1993
Anchorage-dependent Animal Cell Culture by Using a Porous Microcarrier
Bioprocess. Eng. 8, 295-299 1993
Growth of Human Vascular Endothelial Cells on Various Types of Microcarriers
Cytotechnology 13, 203-211 1993
1992
Cultivation of Mammalian Cells on Macroporous Microcarriers
Enzyme. Microb. Technol. 14, 203-208 1992
A Novel Microcarrier Bead Model to Investigate Bone Cell Responses to Mechanical Compressions in Vitro
J Bone Min. Res. 7, Supp. 2, S403-S405 1992
Efficient Serial Propagation of WI38 Cells on Porous Microcarriers(CultiSpher-GL)
Animal Cell Technology: Developments, Processes & Products. Butterworth-Heinemann Ltd. 508-510 1992
Dissolved Oxygen - The Critical Parameter at Cell Growth and Expression of r-Protein in a Porous Microcarrier System
Animal Cell Technology: Developments, Processes & Products. Butterworth-Heinemann Ltd. 212-214 1992
High Density BHK Culture Using Porous Microcarriers
Animal Cell Technology: Developments, Processes & Products. Butterworth-Heinemann Ltd. 480-487 1992
1991
CultiSpher-Macroporous Gelatin Microcarriers- New Applications
In Production of Biologicals from Animal cells in Culture. Butterworth-Heinemann Ltd. 434-438 1991
Comparison of Two Systems Based in Porous Beads for the Production of Recombinant Von Willebrand Factor by CHO Cells
In Production of Biologicals from Animal cells in Culture. Butterworth-Heinemann Ltd. 525-527 1991
Animal Cell Bioreactors - Recent Advances and Challenges to Scale-Up
Canad. J. Chem. Eng. 69, 409-420 1991
Improved Microscopic Observation of Mammalian Cells on Microcarriers by Fluorescent Staining
Cytotechnology 5, 141-146 1991
1990
Production of Recombinant Von Willebrand Factor by CHO Cells Cultured in Macroporous Microcarriers
Cytotechnology 4, 163-171 1990

Biomaterials Aspects of Porous Microcarriers for Animal Cell Culture
TIBTECH 8, 131-136 1990

The Use of Macroporous Gelatin carriers For the Cultivation of Mammalian Cells in Fluidised Bed Reactors
Cytotechnology 3, 271-277 1990
Growth of Cells on Macroporous Microcarriers
In Trends in Animal Cell Culture Technology. VCH Publishers. 35-40, 1990
Immobilization of Animal Cells in Porous Carrier Culture
TIBTECH 8, 204-209 1990
1987
Macroporous Microcarriers
In Modern Approaches to Animal Cell Technology. Butterworth & Co. Ltd. 492-503 1987
1986
Growth of Anchorage Dependent Cells on Macroporous Microcarriers
Bio/Technology 4, 989-990 1986