Application Notes describes the culture of specific lines, production of virus and specific technical solutions.

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Oxygen Limitations in Small Spinner Vessels
Increasing spinner size from 50 to 250 ml reduces cell concentrations 50%.

Large Scale Harvesting of Cells.
More than 90% of the cells are recovered by a modified procedure.

Growth of r-CHO in 1, 4 and 10 litre Fermentors
Production was scaled-up and maintained for more than 2 months.

Critical Parameters for the Cultivation of r-CHO Cells
Expression of recombinant protein is strongly influenced by dissolved oxygen concentration.

Growth of r-CHO Cells in Serum-free Medium
CultiSpher’s porous structure makes long-term serum-free culture possible.

Vesicular Stomatitis Virus(VSV) Production by Vero Cells
Compared to solid microcarriers almost 10 times higher yields of virus were obtained.

Growth of BHK 21 Cells in Medium with Reduced Serum
Growth of BHK 21 cells was maintained at reduced serum levels.

Influence of Dissolved Oxygen Concentration on Cell Growth
Dissolved oxygen is the most critical parameter in spinner cultures.

Optimisation of Microcarrier Concentration
Almost 10 x 10⁹ CHO cells can be grown on 1 g of CultiSpher-G.

Effect of Inoculum Size on Growth Span
Microcarrier cultures with CultiSpher can be scaled-up in steps of 400 times.

Effect of Microcarrier Concentration on Cell Yield
Optimum microcarrier concentration must be determined for each system.

Serial Cultivation of CHO-K1 Cells
Cells harvested from CultiSpher is ideal for scale-up purposes.

Growth of MDCK Cells
MDCK cells can be cultured to high densities and harvested with quantitative yields.

Growth of Recombinant and Non-recombinant CHO-cells
After a prolonged growth phase almost identical cell yields were obtained.

Growth of BHK-21 Cells
BHK cells attached and grew efficiently on CultiSpher-G.

Growth of Vero and GMK Cells
High yields of both these cell lines were obtained. A modified procedure for cell counting must be used.

Growth of HeLa Cells
Large numbers of HeLa cells were produced and collected continuously.